![]() ![]() Several protein segments although known to interact with membrane lipid bilayer have lower average hydrophobicities. Prediction of membrane spanning segments of proteins works well with hydrophobicity calculations ( Kyte and Doolittle, 1982) only for proteins having continuous stretches of peptide segments having significant hydrophobicity. To estimate the hydrophobicity of a protein segment, hydrophobicity of individual amino acids are averaged, assuming that the transfer of a chemical group from an apolar solvent is independent of the larger molecule to which the group is attached. Amino acids with non-polar side chains have higher hydrophobicities compared to amino acids with polar or charged side chains. Hydrophobicity is basically a measure of the degree of affinity between water and the side chain of an amino acid. Hydrophobicity, measured as a free energy change for the transfer of an amino acid from an apolar solvent such as n-octanol to water has been used, in general, to describe protein segments containing non-polar amino acid residues compatible for interaction with lipid bilayer ( Kyte and Doolittle, 1982). Bal Ram Singh, Xu-hai Be, in Techniques in Protein Chemistry III, 1992 II Hydrophobicity and Hydrophobic Moment ![]()
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